R2380-vp - ARID2 (vPairTM) Antibodies

Catalog No. R2380-vp
Product Name ARID2 (vPairTM) Antibodies
Price $299

Predicted | Observed M.W.: 197 I 220 kDa                                                 Uniprot ID: Q68CP9

Application: WB, IP, ChIP

Quantity:        50 ul ARID2 (N) (R2380-1) Rabbit Polyclonal Antibody &

                      50 ul ARID2 (C) (R2380-2) Rabbit Polyclonal Antibody

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.


AT-rich interactive domain-containing protein 2 (ARID2) is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). ARID2 is required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF) and may be involved in targeting the complex to different genes. ARID2 may also be involved in regulating transcriptional activation of cardiac genes.

Other Names:

AT-rich interactive domain-containing protein 2, ARID domain-containing protein 2, BRG1-associated factor 200, BAF200, Zinc finger protein with activation potential, Zipzap/p200

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [ARID2 (N) (R2380-1)]  or the C-terminal [ARID2 (C) (R2380-2)] region of human ARID2. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP & ChIP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

Fig 1. A) Western blot of total cell extracts from WT or 2 independent ARID2-knockout (KO1 and KO2) clones from human hepatoma cell line BEL-7404; using anti-ARID2 (N) (R2380-1) at RT for 2 h. B) Western blot of total cell extracts from a, d. mouse thymus, b. human HeLa, c. human Jurkat; using 2 independent Abs against 2 distinct regions of human ARID2 at RT for 2 h. C) Total extracts from mouse thymus were immunoprecipitated (IP) with IgG or anti-ARID2 (N) (R2380-1) under the conventional IP conditions or cross-linked chromatin immunoprecipitation (ChIP) conditions; followed by WB with anti-ARID2 (C) (R2380-2) at RT for 2 h.