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Predicted I Observed M.W.: 80 kDa                                                            Uniprot ID: Q92841

Application: WB

Quantity:        50 ul DDX17 (N) (R1762-1) Rabbit Polyclonal Antibody &

                      50 ul DDX17 (C) (R1762-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Probable ATP-dependent RNA helicase DDX17 belongs to the DEAD box helicase family and the DDX5/DBP2 subfamily. DDX17 is an RNA-dependent ATPase activity. DDX17 is involved in transcriptional regulation and is a transcriptional coactivator for estrogen receptor ESR1. DDX17 increases ESR1 AF-1 domain-mediated transactivation. Additionally, DDX17 synergizes with DDX5 and SRA1 RNA to activate MYOD1 transcriptional activity and is probably involved in skeletal muscle differentiation. Furthermore, DDX17 is required for zinc-finger antiviral protein ZC3HAV1-mediated mRNA degradation.

Other Names:

Probable ATP-dependent RNA helicase DDX17, DEAD box protein 17, DEAD box protein p72, RNA-dependent helicase p72

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [DDX17 (N) (R1762-1)] or the C-terminal [DDX17 (C) (R1762-2)] region of human DDX17. Antibodies were purified by affinity purification using immunogen. 

Species Specificity:

Human, Mouse

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: