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Predicted I Observed M.W.: 79 I 100 kDa                                                   Uniprot ID: P41182

Application: WB

Quantity:        50 ul BCL6 (N1) (R0107-1) Rabbit Polyclonal Antibody &

                        50 ul BCL6 (N2) (R0107-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

B-cell CLL/lymphoma 6 (BCL6) is a zinc finger transcription factor that acts as a sequence-specific repressor of transcription.  It has been shown to regulate the transcription of STAT-dependent IL-4 responses of B cells.  It contains an N-terminal POZ domain that interacts with other proteins to function as transcription co-repressors.  The gene encoding BCL6 protein has been found to be translocated and mutated in diffuse large-cell lymphoma (DLCL) and may play a role in the pathogenesis of DLCL.                                                                      

Other Names:

B-cell lymphoma 6 protein, B-cell lymphoma 5 protein, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, zinc finger protein 51, BCL5, LAZ3, ZBTB27, ZNF51, BCL6A

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing 2 distinct N-terminal regions of human BCL6.  Antibodies were purified by affinity purification using immunogen. 

Species Specificity:

Human, Mouse

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Recommended Conditions: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: