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Predicted M.W.: 11 kDa                                                                    Uniprot ID: P48298

Application: ELISA

Quantity:       50 ug CCL11 (C) (R0097-1) Rabbit Polyclonal Antibody &

                     50 ug CCL11 (N) (R0097-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Chemokine (C-C motif) ligand 11 (CCL11) is a cytokine with chemotactic activity for eosinophils but not mononuclear cells or neutrophils.  It is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.                 

Other Names:

Scya11, eotaxin 

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the C-terminal region [CCL11(C), R0097-1] or the N-terminal region [CCL11(N), R0097-2] of mouse CCL11.  Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Mouse

Tested Applications: 

ELISA:  1:1,000- 1:10,000 (detect native recombinant protein in a direct ELISA assay). 

              Suitable for using as ELISA capture or detection antibody.