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Quantity: 100 ul                                                                                          Application: WB

Predicted I Observed M.W.: 131 kDa                                                          Uniprot ID: F4IXE7

Background:

IDM1 is a histone H3 acetyltransferase that regulates DNA demethylation in Arabidopsis. IDM1 is capable of recognizing methylated DNA through its MBD domain and recognizing unmethylated histone H3K4 through its PHD domain. IDM1 negatively regulates DNA demethylation, preventing DNA hypermethylation of highly homologous multicopy genes and other repetitive sequences [Qian W. et al., Science 336, 1445 (2012)].

Other Names:

Histone H3 acetyltransferase IDM1, INCREASED DNA METHYLATION 1, REPRESSOR OF SILENCING 4, ROS4

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of arabidopsis thaliana IDM1 (At3g14980). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: