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R3219-1 - IFT74 (N) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB
Predicted I Observed M.W.: 70 kDa Uniprot ID: Q96LB3
Background:
Intraflagellar transport protein 74 homolog (IFT74) is a component of the intraflagellar transport (IFT) complex B: together with IFT81, IFT74 forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. IFT74 binds to beta-tubulin via its basic region, and is required for ciliogenesis.
Other Names:
Intraflagellar transport protein 74 homolog, Capillary morphogenesis gene 1 protein, CMG-1, Coiled-coil domain-containing protein 2, CCDC2, CMG1 Source and Purity:
Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human IFT74. Antibodies were purified by affinity purification using immunogen.
Storage Buffer and Condition:
Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.
Species Specificity:
Human, Mouse
Tested Applications:
WB: 1:1,000-1:3,000 (detect endogenous protein*)
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.
Product Data:
Fig 1. Western blot of total cell extracts fromA, D) mouse thymus, B) human HeLa, C) humanJurkat; using 2 independent Abs against 2 distinctregions of human IFT74 at RT for 2 h. |