R0068-vp - IL25 (vPairTM) Antibodies

Catalog No. R0068-vp
Product Name IL25 (vPairTM) Antibodies
Price $249

Predicted M.W.: 20 kDa                                                        Uniprot ID: Q9H293

Application: WB, ELISA

Quantity:                      50 ug IL25 (N) (R0068-1) Rabbit Polyclonal Antibody &

50 ug IL25 (C) (R0068-2) Rabbit Polyclonal Antibody

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.


Interleukin 25 (IL25) is a cytokine that induces activation of NF-kappa-B and stimulates the production of the proinflammatory chemokine interlukin 8.  IL25 shares sequence similarity with interleukin 17.  It may be a proinflammatory cytokine favoring Th2-type immune response.  IL25 along with interleukin 17B are ligands for the cytokine receptor IL17BR             

Other Names:

UNQ312/PRO10272, IL17E

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [IL25 (N) (R0068-1)] or the C-terminal [IL25 (C) (R0068-2)] region of human IL25. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:


Tested Applications: 

WB: 1:1,000-1:5,000 (detect recombinant protein)

ELISA:  1:1,000 – 1:10,000 (detect native recombinant protein in a direct ELISA assay)

             Suitable for using as ELISA capture or detection antibodies.

Product Data:

IL25 Antibody Western (Abiocode)

Fig 1. Western blot of recombinant human
IL-25 protein (A: 10ng, B: 60 ng), using Ab
(R0068-1) at RT for 2 h.