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Quantity: 100 ul                                                                                         Application: WB

Predicted I Observed M.W.: 110 I 125 kDa                                                 Uniprot ID: Q84VW9

Background:

Phosphoenolpyruvate carboxylase (PEPC) is also known as Phosphoenolpyruvate carboxylase 3 (PPC3), which is a cytosolic PEPC that has activity when expressed in E.coli. Its mRNA is most abundantly expressed in roots and siliques. PEPC belongs to the plant-type PEPC family. It can form an enzymatically active complex with a castor bean ortholog of PPC4, which encodes a bacterial-type PEPC. PEPC is often used as a marker for the cytoplasmic protein fraction.

Other Names:

Phosphoenolpyruvate carboxylase 3, AtPPC3, PEPC 3, PEPCase 3, PPC3, PPC

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of arabidopsis thaliana PEPC (At3g14940). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis thaliana

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: