R2489-vp - ACTN1 (vPairTM) Antibodies

Catalog No. R2489-vp
Product Name ACTN1 (vPairTM) Antibodies
Price $249

Predicted I Observed M.W.: 103 kDa                                                          Uniprot ID: P12814

Application: WB

Quantity:        50 ul ACTN1 (M) (R2489-1) Rabbit Polyclonal Antibody &

                      50 ul ACTN1 (C) (R2489-2) Rabbit Polyclonal Antibody

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.


Alpha-actinin-1 (ACTN1) is a bundling protein belonging to the alpha-actinin family. ACTN1 is an F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures.

Other Names:

Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the middle [ACTN1 (M) (R2489-1)] or the C-terminal [ACTN1 (C) (R2489-2)] region of human ACTN1. Antibodies were purified by affinity purification using immunogen.

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

ACTN1 Antibody Western (Abiocode)

Fig 1.  Western blot of total cell extracts from A, E) mouse
brain, B, F) mouse thymus, C, G) human HeLa, D, H) human
Jurkat; using 2 independent Abs against 2 distinct regions of
human ACTN1 at RT for 2 h.