Products

R2906-vp - DSP (vPairTM) Antibodies

Catalog No. R2906-vp
Product Name DSP (vPairTM) Antibodies
Price $279
  
 

Predicted I Observed M.W.: 332 kDa                                                          Uniprot ID: P15924

Application: WB

Quantity:        50 ul DSP (N) (R2906-1) Rabbit Polyclonal Antibody &

                      50 ul DSP (C) (R2906-2) Rabbit Polyclonal Antibody 


Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Desmoplakin (DSP) belongs to the plakin or cytolinker family. DSP is a major high molecular weight protein of desmosomes. DSP is involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.

Other Names:

Desmoplakin, DP, 250/210 kDa paraneoplastic pemphigus antigen

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [DSP (N) (R2906-1)] or the C-terminal [DSP (C) (R2906-2)] region of human DSP. Antibodies were purified by affinity purification using immunogen.

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

DSP Antibody Western (Abiocode)

Fig 1.  Western blot of total cell extracts
from human HeLa, using A) anti-DSP (N)
(R2906-1); B) anti-DSP (C) (R2906-2) at
RT for 2 h.