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Quantity: 100 ul                                                                                    Application: WB

Predicted I Observed M.W.: 61 kDa                                                      Uniprot ID: Q9S745

Background:

PAD4 is a probable lipase required downstream of MPK4 for accumulation of the plant defense-potentiating molecule, salicylic acid (SA), thus contributing to the plant innate immunity against invasive biotrophic pathogens and to defense mechanisms upon recognition of microbe-associated molecular patterns (MAMPs). PAD4 is necessary for the salicylic acid-(SA-) dependent systemic acquired resistance (SAR) response that involves expression of multiple defense responses. PAD4 may also function in response to abiotic stresses such as UV-C light and LSD1-dependent acclimatization to light conditions that promote excess excitation energy (EEE), probably by transducing redox signals and modulating stomatal conductance.

Other Names:

Lipase-like PAD4, Protein ENHANCED DISEASE SUSCEPTIBILITY 9, Protein PHYTOALEXIN DEFICIENT 4, AtPAD4, EDS9, At3g52430

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing C-terminal region of arabidopsis thaliana PAD4 (AT3G52430). Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Arabidopsis

Tested Applications: 

WB: 1:500-1:2,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: