L3204-J500 - Human Jurkat Cell Extracts

Catalog No. L3204-J500
Product Name Human Jurkat Cell Extracts
Price $49

Application: WB

Quantity: 500 ug in 100 ul, 5 ug/ul

Product Description:

Protein extracts were prepared from human Jurkat cells by lyzing cell pellets in a buffer containing 50 mM Tris, pH 8; 0.4 M NaCl; 0.5% NP-40; 10% Glycerol and 1 mM EDTA, supplemented freshly with 1 mM DTT and 1 mM PMSF.  After incubating on ice for 20 min, protein lysates were centrifuged at 13,000 rpm at 4oC for 5 min, and supernatants were transferred to fresh tubes. The protein concentration was determined by the Bradford protein assay. The protein extract was standardized to 5 ug/ul with 2 x SDS sample buffer, followed by denaturation at 95°C for 5 min.

Storage Condition:

Keep at -20°C. Stable for 6 months from date of receipt.

Usage Instruction: 

For Western blot analysis, incubate protein extracts at 95°C for 5 min and load 8 - 10 ul of the extract per lane.