R0268-vp - BHLHE40 (vPairTM) Antibodies

Catalog No. R0268-vp
Product Name BHLHE40 (vPairTM) Antibodies
Price $249

Predicted M.W.: 45 kDa                                                                                Uniprot ID: O35185

Application: WB

Quantity:        50 ul BHLHE40 (N) (R0268-1) Rabbit Polyclonal Antibody &

                      50 ul BHLHE40 (C) (R0268-2) Rabbit Polyclonal Antibody

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.


BHLHE40 is a basic helix-loop-helix protein expressed in various tissues and is induced by Bt2cAMP in chondrocytes. BHLHE40 is believed to be involved in the control of cell differentiation. It may function as a transcriptional factor to modulate chondrogenesis and neuronal differentiation. BHLHE40 Interacts with ubiquitin-conjugating enzyme UBE2I/UBC9, which may lead to proteasomal degradation.

Other Names:

basic helix-loop-helix family, member e40, SHARP-2, Class E basic helix-loop-helix protein 40, bHLHe40, Class B basic helix-loop-helix protein 2, bHLHb2, E47 interaction protein 1, EIP1, Stimulated by retinoic acid gene 13 protein, Bhlhb2, Clast5, Stra13, BHE40

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [BHLHE40 (N) (R0268-1)] or the C-terminal [BHLHE40 (C) (R0268-2)] regions of mouse BHLHE40. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

BHLHE40 Antibody Western (Abiocode)

Fig 1. Western blot of total cell extracts from human
MCF7, using 2 independent Abs against 2 distinct
regions of mouse BHLHE40 [A: R0268-1 (N-terminal);
B: R0268-2 (C-terminal)] at RT for 2 h.