R0191-vp - PARP (vPairTM) Antibodies

Catalog No. R0191-vp
Product Name PARP (vPairTM) Antibodies
Price $249

Predicted M.W.: 113 kDa                                                                              Uniprot ID: P09874

Application: WB

Quantity:        50 ul PARP (N) (R0191-1) Rabbit Polyclonal Antibody &

                      50 ul PARP (M) (R0191-2) Rabbit Polyclonal Antibody

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.


Poly [ADP-ribose] polymerase 1 (PARP) is involved in the base excision repair (BER) pathway by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.

Other Names:

ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, PARP-1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [PARP (N) (R0191-1)] or the middle [PARP (M) (R0191-2)] region of human PARP. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:


Tested Applications: 

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

PARP Antibody Western (Abiocode)

Fig 1. Western blot of total cell extracts from human
Jurkat, using 2 independent Abs against 2 distinct
regions of human PARP [A: R0191-1 (N-terminal);
B: R0191-2 (middle)] at RT for 2 h.