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Uniprot ID: Q99MZ3                                                                           Application: WB, IP, ChIP

Predicted I Observed M.W.: 95 I 120 kDa  

Quantity:       50 ul MLXIPL (N2) (R2556-2) Rabbit Polyclonal Antibody &

                     50 ul MLXIPL (C) (R2556-3) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

MLX-interacting protein-like (MLXIPL) is a nuclear protein involved in transcriptional regulation. MLXIPL contains 1 basic helix-loop-helix (bHLH) domain and binds to the canonical and non-canonical E box sequences 5'-CACGTG-3'. MLXIPL is a transcriptional repressor.

Other Names:

Carbohydrate-responsive element-binding protein, ChREBP, MLX interactor, MLX-interacting protein-like, Williams-Beuren syndrome chromosomal region 14 protein homolog, Mio, Wbscr14

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [MLXIPL (N2) (R2556-2)] or the C-terminal [MLXIPL (C) (R2556-3)] region of mouse MLXIPL. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP & ChIP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: