R0008-vp - beta-Catenin1 (vPairTM) Antibodies

Catalog No. R0008-vp
Product Name beta-Catenin1 (vPairTM) Antibodies
Price $249

Predicted M.W.: 85 kDa                                                        Uniprot ID: P35222

Application: WB

Quantity:          50 ul b-catenin1 (N) (R0008-1) Rabbit Polyclonal Antibody &

                          50 ul b-catenin1 (C) (R0008-2) Rabbit Polyclonal Antibody

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.


β-catenin1 is a key downstream effector of the Wnt signaling pathway. It is implicated in early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin1 on Ser45, which primes β-catenin1 for subsequent phosphorylation by GSK-3 at Ser33, Ser37 and Thr41. Phosphorylation of β-catenin1 by GSK-3β leads to its degradation. Wnt signaling antagonizes GSK-3b and stablizes b-catenin1. Mutations in these phosphorylation sites, which result in the stabilization of β-catenin1 protein levels, have been found in many tumor cell lines.

Other Names:

beta-catenin, CTNNB1, OK/SW-cl.35, CATNB, DKFZp686D02253, FLJ25606, FLJ37923

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal [b-catenin1 (N) (R0008-1)] or the C-terminal region [b-catenin1 (C) (R0008-2)] of human b-catenin1. Antibodies were purified by affinity purification using immunogens. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

beta-catenin Antibody Western (Abiocode)

Fig 1.  Western blot of total cell extracts from
human MDA-MB231 (A, D), mouse brain (B, E)
and mouse thymus (C, F), using indicated
antibodies at RT for 2 h.