Abiocode Logo

Quantity: 100 ul                                                                                             Application: WB

Predicted I Observed M.W.: 108 I 120 kDa                                                 Uniprot ID: P19021

Background:

Peptidyl-glycine alpha-amidating monooxygenase (PAM) is a bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity. Several PAM isoforms have been identified. Isoform 1 and 2 are single-pass type I membrane proteins, while isoform 3 and 4 are secreted.

Other Names:

Peptidyl-glycine alpha-amidating monooxygenase, PAM

PAM contains 2 domains: Peptidylglycine alpha-hydroxylating monooxygenase (PHM)
and Peptidyl-alpha-hydroxyglycine alpha-amidating lyase or Peptidylamidoglycolate lyase (PAL)

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of human PAM. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: