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Predicted I Observed M.W.: 100 kDa                                                           Uniprot ID: Q96AV8

Application: WB, IP

Quantity:        50 ul E2F7 (N) (R2593-1) Rabbit Polyclonal Antibody &

                      50 ul E2F7 (C) (R2593-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

E2F7 is an atypical E2F transcription factor that participates in various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. E2F7 mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. E2F7 directly represses transcription of classical E2F transcription factors such as E2F1. E2F7 also acts as a regulator of S-phase by recognizing and binding the E2-related site 5'-TTCCCGCC-3' and mediating repression of G1/S-regulated genes. E2F7 plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). E2F7 is required for placental development by promoting polyploidization of trophoblast giant cells. E2F7 is also involved in DNA damage response: it is up-regulated by p53/TP53 following genotoxic stress and acts as a downstream effector of p53/TP53-dependent repression by mediating repression of indirect p53/TP53 target genes involved in DNA replication.

Other Names:

Transcription factor E2F7, E2F-7

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [E2F7 (N) (R2593-1)] or the C-terminal [E2F7 (C) (R2593-2)] region of human E2F7. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP: 1:100 - 1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Species Specificity:

Human

Product Data: