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R0795-vp - EBF4 (vPairTM) Antibodies
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Predicted I Observed M.W.: 64, 68 I 60, 68 kDa Uniprot ID: Q9BQW3 Application: WB Quantity: 50 ul EBF4 (C2) (R0795-2) Rabbit Polyclonal Antibody & 50 ul EBF4 (C3) (R0795-3) Rabbit Polyclonal Antibody Product Introduction: vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Background: Transcription factor Early B-cell factor 4 (EBF4) is also known as COE4, which belongs to the conserved Olf/EBF family of helix-loop-helix transcription factors, members of which play important roles in neural development and B-cell maturation. EBF4 recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Other Names: Transcription factor COE4, Early B-cell factor 4, EBF-4, Olf-1/EBF-like 4, O/E-4, OE-4, COE4, KIAA1442 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing rabbit with GST-fusion proteins containing two distinct C-terminal regions of human EBF4. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human, Mouse Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data:
(B) mouse thymus, (C) human HeLa, (D, F) human Jurkat; using 2 independent Abs against 2 distinct regions of human EBF4 at RT for 2 h. The 60 kD and 68 kD bands may represent different isoforms of EBF4 differentially expressed in various tissues/cells. |
