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Predicted I Observed M.W.: 81 I 100 kDa                                                   Uniprot ID: Q8TBB1 

Application: WB

Quantity:        50 ul LNX1 (N) (R1715-1) Rabbit Polyclonal Antibody &

                      50 ul LNX1 (C) (R1715-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

LNX1 mediates ubiquitination and subsequent proteasomal degradation of NUMB. LNX1 accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. LNX1 mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65.

Other Names:

E3 ubiquitin-protein ligase LNX, Ligand of Numb-protein X 1, Numb-binding protein 1, PDZ domain-containing RING finger protein 2, LNX, PDZRN2, MPDZ

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [LNX1 (N) (R1715-1)] or the C-terminal [LNX1 (C) (R1715-2)] region of human LNX1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

LNX1 (N) (R1715-1): Human, Mouse

LNX1 (C) (R1715-2): Human

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: