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Predicted I Observed M.W.: 134 kDa                                                          Uniprot ID: P48357 

Application: WB

Quantity:        50 ul LEPR (M) (R2605-1) Rabbit Polyclonal Antibody &

                      50 ul LEPR (C) (R2605-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

LEPR is the receptor for obesity factor (leptin). On ligand binding, LEPR mediates signaling through JAK2/STAT3. LEPR is involved in the regulation of fat metabolism, and in a hematopoietic pathway, LEPR is required for normal lymphopoiesis. LEPR may also play a role in reproduction. LEPR can also mediate the ERK/FOS signaling pathway.

Other Names:

Leptin receptor, LEP-R, HuB219, OB receptor, OB-R, CD295, DB, OBR

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the middle [LEPR (M) (R2605-1)] or the C-terminal region of human LEPR. Antibodies were purified by affinity purification using immunogen.  

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: