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Quantity: 100 ul                                                                                         Application: WB

Predicted I Observed M.W.: 96 kDa                                                           Uniprot ID: Q9Y2X0

Background:

MED16 component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED16 functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED16 is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Other Names:

Mediator of RNA polymerase II transcription subunit 16, Mediator complex subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, Trap95, Vitamin D3 receptor-interacting protein complex 92 kDa component, DRIP92, DRIP92, THRAP5

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of human MED16. Antibodies were purified by affinity purification using immunogen.

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: