Products

R1967-vp - LHX2 (vPairTM) Antibodies

Catalog No. R1967-vp
Product Name LHX2 (vPairTM) Antibodies
Price $249
  
 

Predicted | Observed M.W.: 44 I 58 kDa                                                     Uniprot ID: P50458

Application: WB

Quantity:        50 ul LHX2 (N) (R1967-1) Rabbit Polyclonal Antibody &

                       50 ul LHX2 (C) (R1967-2) Rabbit Polyclonal Antibody


Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

LIM/homeobox protein Lhx2 (LHX2) acts as a transcriptional activator. LIM-Hox genes function to stimulate the promoter of the alpha-glycoprotein gene. They are transcriptional regulatory proteins involved in the control of cell differentiation in developing lymphoid and neural cell types.

Other Names:

LIM/homeobox protein Lhx2, Homeobox protein LH-2, LIM homeobox protein 2, LH2

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [LHX2 (N) (R1967-1)] or the C-terminal [LHX2 (C) (R1967-2)] region of human LHX2. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

LHX2 (N) (R1967-1): Human, Mouse

LHX2 (C) (R1967-2): Human

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

LHX2 Antibody Western (Abiocode)

Fig 1. Western blot of total cell extracts from
(A) mouse thymus, (B, D) human HeLa, (C, E)
human Jurkat; using 2 independent Abs against
2 distinct regions of human LHX2 at RT for 2 h.