Abiocode Logo
Products
Contact Us
- Telephone:
1-818-707-0309 - E-Mail:
Abiocode@Abiocode.com
M1092-4a - TET3 (N2) Antibody, Mouse Monoclonal
|
Application: WB, Flow cyt Quantity: 100 ug Predicted M.W.: 180 kDa Uniprot ID: O43151 Isotype: Mouse IgG1 Clone: 2B7 Background: TET3 is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), and may play a role in DNA demethylation. TET3 is a member of the ten-eleven translocation (TET) gene family. Other Names: Methylcytosine dioxygenase TET3, KIAA0401 Source and Purity: Mouse monoclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of mouse TET3. Antibodies were purified by Caprylic Acid- Ammonium Sulfate precipitation. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 40% Glycerol, 0.02% Thimerosal. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human, Mouse Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) Flow cytometry: 1:100-1:400
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. A) Western blot of total cell extracts from human 293 T cells transfected with the empty vector, or Flag-tagged murine TET1, TET2 or TET3; using indicated Abs at RT for 2h. B) Western blot of total cell extracts from a. mouse brain, b. mouse thymus, c. human Jurkat; using monoclonal anti-TET3 (N2) (M1092-4a) at RT for 2 h. C) Flow cytometry was performed with thymocytes or Jurkat cells, using PE-labeled 2’ anti-mouse IgG only (black line), or anti-TET3 (N2) (M1092-4a) followed by 2’ anti-mouse IgG-PE (red line). |