Products

M1092-4a - TET3 (N2) Antibody, Mouse Monoclonal

Catalog No. M1092-4a
Product Name TET3 (N2) Antibody, Mouse Monoclonal
Price $279
  
 

Application: WB, Flow cyt                                                                        Quantity: 100 ug

Predicted M.W.: 180 kDa                                                                           Uniprot ID: O43151

Isotype: Mouse IgG1                                                                                  Clone: 2B7


Background:

TET3 is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), and may play a role in DNA demethylation. TET3 is a member of the ten-eleven translocation (TET) gene family.

Other Names:

Methylcytosine dioxygenase TET3, KIAA0401

Source and Purity:

Mouse monoclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of mouse TET3. Antibodies were purified by Caprylic Acid- Ammonium Sulfate precipitation. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 40% Glycerol, 0.02% Thimerosal. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

Flow cytometry: 1:100-1:400

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications. 

Product Data:

TET3 Antibody Western (Abiocode)

Fig 1. A) Western blot of total cell extracts from human 293 T cells transfected with the empty vector, or Flag-tagged murine TET1, TET2 or TET3; using indicated Abs at RT for 2h. B) Western blot of total cell extracts from a. mouse brain, b. mouse thymus, c. human Jurkat; using monoclonal anti-TET3 (N2) (M1092-4a) at RT for 2 h. C) Flow cytometry was performed with thymocytes or Jurkat cells, using PE-labeled 2’ anti-mouse IgG only (black line), or anti-TET3 (N2) (M1092-4a) followed by 2’ anti-mouse IgG-PE (red line).