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Predicted I Observed M.W.: 101 kDa                                                          Uniprot ID: Q9ULT6

Application: WB, IP

Quantity:        50 ul ZNRF3 (N) (R2407-1) Rabbit Polyclonal Antibody &

                      50 ul ZNRF3 (C) (R2407-2) Rabbit Polyclonal Antibody

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Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

E3 ubiquitin-protein ligase ZNRF3 is a single-pass type I membrane protein belonging to the ZNRF3 family. ZNRF3 is an E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. ZNRF3 acts on both canonical and non-canonical Wnt signaling pathway. Additionally, ZNRF3 acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.

Other Names:

E3 ubiquitin-protein ligase ZNRF3, RING finger protein 203, Zinc/RING finger protein 3, KIAA1133, RNF203

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [ZNRF3 (N) (R2407-1)] or the C-terminal [ZNRF3 (C) (R2407-2)] region of human ZNRF3. Antibodies were purified by affinity purification using immunogen. 

Species Specificity:

Human, Mouse

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

Fig 1. (A) Western blot of total cell extracts from a. mouse thymus, b,d. human HeLa, c,e. human Jurkat; using 2 independent Abs against 2 distinct regions of human ZNRF3 at RT for 2 h. (B) IP-WB. Immunoprecipitation (IP) was performed with human Jurkat extracts with IgG or anti-ZNRF3 (C) (R2407-2), followed by WB with anti-ZNRF3 (N) (R2407-1).