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R2513-3a - RNF43 (C2) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB, IP
Predicted I Observed M.W.: 86 I 90, 130 kDa Uniprot ID: Q5NCP0
Background:
E3 ubiquitin-protein ligase RNF43 is a single-pass type I membrane protein belonging to the ZNRF3 family. RNF43 is an E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. RNF43 acts on both canonical and non-canonical Wnt signaling pathway, as well as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.
Other Names:
E3 ubiquitin-protein ligase RNF43, RING finger protein 43
Source and Purity:
Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of mouse RNF43. Antibodies were purified by affinity purification using immunogen.
Storage Buffer and Condition:
Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.
Species Specificity:
Human, Mouse
Tested Applications:
WB: 1:1,000-1:3,000 (detect endogenous protein*) IP: 1:100-1:200 *: The apparent protein size on WB may be different from the calculated M.W. due to modifications.
Product Data: Fig 1. (A) Western blot of total cell extracts from a. human HeLa, b, c. human Jurkat; using 2 independent Abs against 2 distinct regions of human or mouse RNF43 at RT for 2 h. These Abs recognize the 90 kD full-length RNF43 and a 130 kD protein, possibly a modified form of RNF43. (B) IP-WB. Immunoprecipitation (IP) was performed with human HeLa extracts with IgG or anti-RNF43 (C2) (R2513-3a), followed by WB with anti-RNF43 (N) (R2513-1). Only the full length RNF43, but not the modified form, was immunoprecipitated.
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