Abiocode Logo

Predicted I Observed M.W.: 27 kDa                                                             Uniprot ID: P42212

Application: WB

Quantity:        50 ul GFP (N2) (M0802-3a) Mouse Monoclonal (Isotype: Mouse IgG1; Clone: 6F6)

                      50 ul GFP (M) (M0802-4a) Mouse Monoclonal (Isotype: Mouse IgG1; Clone: 9A12)

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Green fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins in living cells without the need for chemical staining. YFP differs from GFP due to a T203Y mutation; antibodies raised against GFP should also detect YFP.

Other Names:

Green fluorescent protein

Source and Purity:

Mouse monoclonal antibodies were produced by immunizing animals with GST fusion proteins containing either the N-terminal [GFP (N2) (M0802-3a)] or the middle [GFP (M) (M0802-4a)] region of GFP from Aequorea victoria (Jellyfish). Antibodies were purified by Caprylic Acid- Ammonium Sulfate precipitation. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 40% Glycerol, 0.02% Thimerosal. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

This anti-GFP antibody recognizes the enhanced form of GFP (EGFP), GFP and yellow FP (YFP) made by Aequorea Victoria (Jellyfish).

Tested Applications:

WB: 1:1,000-1:3,000

Product Data: