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Predicted I Observed M.W.: 69 I 75 kDa                                                     Uniprot ID: O95671

Application: WB

Quantity:        50 ul ASMTL (N) (R0205-1) Rabbit Polyclonal Antibody &

                        50 ul ASMTL (C) (R0205-2) Rabbit Polyclonal Antibody

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

The ASMTL protein has an N-terminus that is similar to the multicopy associated filamentation (maf) protein of Bacillus subtilis and to orfE of Escherichia coli, while the C-terminus is similar to N-acetylserotonin O-methyltransferase. This ASMTL gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. Three transcript variants encoding different isoforms have been found for this gene. The function of the ASMTL protein is Unknown. The presence of the putative catalytic domain of S-adenosyl-L-methionine binding argues for a methyltransferase activity (provided by RefSeq and Uniprot)

Other Names:

N-acetylserotonin O-methyltransferase-like protein, acetylserotonin O-methyltransferase-like, ASMTLX, ASMTLY

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [ASMTL (N) (R0205-1)] or the C-terminal [ASMTL (C) (R0205-2)] region of human ASMTL. Antibodies were purified by affinity purification using immunogen. 

Species Specificity:

Human, Mouse

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: