Products

R0939-vp - HECTD3 (vPairTM) Antibodies

Catalog No. R0939-vp
Product Name HECTD3 (vPairTM) Antibodies
Price $279
  
 

Predicted I Observed M.W.: 97 kDa                                                            Uniprot ID: Q5T447

Application: WB

Quantity:        50 ul IKZF3 (N) (R0793-1) Rabbit Polyclonal Antibody &

                        50 ul IKZF3 (C1) (R0793-2) Rabbit Polyclonal Antibody 


Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

E3 ubiquitin-protein ligase HECTD3 accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. HECTD3 mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP.

Other Names:

E3 ubiquitin-protein ligase HECTD3, HECT domain-containing protein 3, HECTD3

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [HECTD3 (N) (R0939-1)] or the C-terminal [HECTD3 (C) (R0939-2)] region of human HECTD3. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

HECTD3 Antibody Western (Abiocode)

Fig 1. Western blot of total cell extracts from
(A, C) human HeLa, (B) human Jurkat; using
2 independent Abs against 2 distinct regions
of human HECTD3 at RT for 2 h.