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Predicted M.W.: 133 kDa                                                                  Uniprot ID: Q8TAQ2

Application: WB

Quantity:         50 ul SMARCC2 (M) (R0012-1) Rabbit Polyclonal Antibody &

                       50 ul SMARCC2 (C) (R0012-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

A member of the SWI/SNF family of proteins, SMARCC2 is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).  SMARCC2 can stimulate the ATPase activity of the catalytic subunit of these complexes. 

Other Names:

BAF170, CRACC2, DKFZp313D0632, Rsc8

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the middle region [SMARCC2 (M) (R0012-1)] or the C-terminal region [SMARCC2 (C) (R0012-2)] of human SMARCC2. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:5,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: