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Predicted I Observed M.W.: 43 I 62 kDa                                                     Uniprot ID: O43435

Application: WB

Quantity:        50 ul TBX1 (N) (R1797-1) Rabbit Polyclonal Antibody &

                       50 ul TBX1 (C) (R1797-2) Rabbit Polyclonal Antibody

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Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

T-box transcription factor TBX1 is a probable transcriptional regulator involved in developmental processes. TBX1 is required for normal development of the pharyngeal arch arteries.

Other Names:

T-box transcription factor TBX1, T-box protein 1, Testis-specific T-box protein

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [TBX1 (N) (R1797-1)] or the C-terminal [TBX1 (C) (R1797-2)] region of human TBX1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

Fig 1. Western blot of total cell extracts from

(A, B) human HeLa; using 2 independent Abs

against 2 distinct regions of human TBX1

at RT for 2 h.