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Quantity: 100 ul                                                                                          Application: WB

Predicted I Observed M.W.: 101 kDa                                                          Uniprot ID: Q12967

Quantity:        50 ul RALGDS (N) (R1857-1) Rabbit Polyclonal Antibody &

                        50 ul RALGDS (C) (R1857-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Ral guanine nucleotide dissociation stimulator (RALGDS ) stimulates the dissociation of GDP from the Ras-related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Furthermore, RALGDS interacts and acts as an effector molecule for R-Ras, H-Ras, K-Ras, and Rap.

Other Names:

Ral guanine nucleotide dissociation stimulator, RalGDS, Ral guanine nucleotide exchange factor, RalGEF

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [RALGDS (N) (R1857-1)] or the C-terminal [RALGDS (C) (R1857-2)] region of human RALGDS. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: