Abiocode Logo

Predicted M.W.: 17 kDa                                                        Uniprot ID: P05112

Application: WB, ELISA

Quantity:        50 ug IL4 (N) (R0071-1) Rabbit Polyclonal Antibody &

                      50 ug IL4 (C) (R0071-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Interleukin 4 (IL4) is a pleiotropic cytokine produced by activated T cells.  It participates in several B-cell activation processes as well as of other cell types.  It induces the expression of class II MHC molecules on resting B-cells.  IL4 is the ligand for interleukin 4 receptor which can also bind IL13.  IL4 is a costimulator of DNA-synthesis.  It enhances secretion and cell surface expression of IgE and IgG1.      

Other Names:

BCGF-1, BCGF1, BSF1, IL-4, MGC79402

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing either the N-terminal region [IL4 (N) (R0071-1)] or the C-terminal region [IL4 (C) (R0071-2)] of human IL4. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Recommended Conditions: 

WB: 1:1,000-1:5,000 (detect recombinant protein)

ELISA:  > 1:1,000- 1:10,000 (detect native recombinant protein in a direct ELISA assay). Suitable for using as ELISA capture or detection antibody.

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: