Abiocode Logo

Predicted I Observed M.W.: 84 I 100 kDa                                                   Uniprot ID: Q14244

Application: WB

Quantity:        50 ul MAP7 (N) (R2180-1) Rabbit Polyclonal Antibody &

                       50 ul MAP7 (C) (R2180-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Ensconsin (MAP7) belongs to the MAP7 family and is a microtubule-stabilizing protein that may play an important role during reorganization of microtubules during polarization and differentiation of epithelial cells. MAP7 associates with microtubules in a dynamic manner and may play a role in the formation of intercellular contacts. MAP7 colocalization with TRPV4 results in the redistribution of TRPV4 toward the membrane and may link cytoskeletal microfilaments.

Other Names:

Ensconsin, Epithelial microtubule-associated protein of 115 kDa, E-MAP-115, Microtubule-associated protein 7, MAP-7

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [MAP7 (N) (R2180-1)] or the C-terminal [MAP7 (C) (R2180-2)] region of human MAP7. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: