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Predicted | Observed M.W.: 105 I 135 kDa                                                  Uniprot ID: P22670

Application: WB, IP, ChIP

Quantity:        50 ul RFX1 (N) (R2972-1) Rabbit Polyclonal Antibody &

                      50 ul RFX1 (C) (R2972-2) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Regulatory factor X 1 (RFX1) is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX1 is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. RFX1 binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat required for expression of hepatitis B virus genes [provided by RefSeq].

Other Names:

MHC class II regulatory factor RFX1, Enhancer factor C, EF-C, Regulatory factor X 1, RFX, Transcription factor RFX1

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [RFX1 (N) (R2972-1)] or the C-terminal [RFX1 (C) (R2972-2)] region of human RFX1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP & ChIP:1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: