Abiocode Logo

Predicted | Observed MW: 242 I 270 kDa                                                   Uniprot ID: O14497

Application: WB, IP

Quantity:        50 ul ARID1A (N2) (R0436-2) Rabbit Polyclonal Antibody &

                      50 ul ARID1A (C) (R0436-3) Rabbit Polyclonal Antibody 

Product Introduction: 

 vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

AT-rich interactive domain-containing protein 1A (ARID1A) is a member of the SWI/SNF family, which have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for ARID1A [provided by RefSeq].

Other Names:

AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A, B120, BRG1-associated factor 250, BAF250, BRG1-associated factor 250a, BAF250A, Osa homolog 1, hOSA1, SWI-like protein, SWI/SNF complex protein p270, SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1, hELD, BAF250, BAF250A, C1orf4, OSA1, SMARCF1

Species Specificity:

Human, Mouse

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [ARID1A (N2) (R0436-2)] or the C-terminal [ARID1A (C) (R0436-3)] region of human ARID1A. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: