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Predicted I Observed M.W.: 103 kDa                                                         Uniprot ID: O95049

Application: WB

Quantity:        50 ul TJP3 (N) (R1671-1) Rabbit Polyclonal Antibody &

                      50 ul TJP3 (C) (R1671-2a) Rabbit Polyclonal Antibody

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Tight junction protein 3 (TJP3) is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. TJP3 plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms of TJP3 [provided by RefSeq].

Other Names:

Tight junction protein ZO-3, Tight junction protein 3, Zona occludens protein 3, Zonula occludens protein 3, ZO3

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion protein containing either the N-terminal [TJP3 (N) (R1671-1)] or the C-terminal [TJP3 (C) (R1671-2a)] region of human TJP3. Antibodies were purified by affinity purification using immunogen. 

Species Specificity:

Human

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: