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Predicted I Observed M.W.: 87 kDa                                                            Uniprot ID: P18074

Application: WB

Quantity:        50 ul ERCC2 (N) (R1594-1) Rabbit Polyclonal Antibody &

                        50 ul ERCC2 (C) (R1594-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. DNA excision repair protein ERCC2 is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. ERCC2 has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in ERCC2 can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene [provided by RefSeq].

Other Names:

TFIIH basal transcription factor complex helicase XPD subunit, Basic transcription factor 2 80 kDa subunit, BTF2 p80, CXPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells, TFIIH basal transcription factor complex 80 kDa subunit, TFIIH 80 kDa subunit, TFIIH p80, Xeroderma pigmentosum group D-complementing protein, EM9, MAG, MGC102762, MGC126218, MGC126219, XPD

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [ERCC2 (N) (R1594-1)] or the C-terminal [ERCC2 (C) (R1594-2)] region of human ERCC2. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: