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Predicted I Observed M.W.: 88 I 100 kDa                                                   Uniprot ID: P55287

Application: WB, IP

Quantity:        50 ul CDH11 (M) (R2384-1) Rabbit Polyclonal Antibody &

                        50 ul CDH11 (C) (R2384-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Cadherin-11 (CDH11) is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of CDH11 in osteoblastic cell lines and its upregulation during differentiation suggest a specific function of CDH11 in bone development and maintenance [provided by RefSeq].

Other Names:

Cadherin-11, OSF-4, Osteoblast cadherin, OB-cadherin, CAD11, OB

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the middle [CDH11 (M) (R2384-1)] or the C-terminal [CDH11 (C) (R2384-2)] region of human CDH11. Antibodies were purified by affinity purification using immunogen. 

Species Specificity:

Human, Mouse

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: