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Predicted I Observed M.W.: 87 I 120, 75 kDa                                             Uniprot ID: P16473

Application: WB, IP

Quantity:        50 ul TSHR (M) (R2806-1) Rabbit Polyclonal Antibody &

                      50 ul TSHR (C) (R2806-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Thyroid-stimulating hormone receptor (TSHR) is a membrane protein and a major controller of thyroid cell metabolism. TSHR is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in the TSHR gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene [provided by RefSeq].

Other Names:

Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R, LGR3

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the middle [TSHR (M) (R2806-1)] or the C-terminal [TSHR (C) (R2806-2)] region of human TSHR. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: