Abiocode Logo

Predicted I Observed M.W.: 95 I 140 kDa                                                   Uniprot ID: P13591

Application: WB

Quantity:        50 ul NCAM1 (N) (R2444-1) Rabbit Polyclonal Antibody &

                      50 ul NCAM1 (C) (R2444-2) Rabbit Polyclonal Antibody  

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

NCAM1 is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. NCAM1 has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.

Other Names:

Neural cell adhesion molecule 1, N-CAM-1, NCAM-1, CD_antigen=CD56, NCAM

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either N-terminal [NCAM1 (N) (R2444-1)] or the C-terminal [NCAM1 (C) (R2444-2)] region of human NCAM1. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

 WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: