Products

R2914-vp - SEPT9 (vPairTM) Antibodies

Catalog No. R2914-vp
Product Name SEPT9 (vPairTM) Antibodies
Price $279
  
 

Predicted I Observed M.W.: 65 I 80 kDa                                                     Uniprot ID: Q9UHD8

Application: WB

Quantity:        50 ul SEPT9 (N) (R2914-1) Rabbit Polyclonal Antibody &

                      50 ul SEPT9 (C) (R2914-2) Rabbit Polyclonal Antibody 


Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

SEPT9 is a filament-forming cytoskeletal GTPase. SEPT9 may play a role in cytokinesis. SEPT9 may play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Other Names:

Septin-, MLL septin-like fusion protein, MSF-A, MLL septin-like fusion protein, Ovarian/Breast septin, Ov/Br septin, Septin D1, KIAA0991, MSF

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [SEPT9 (N) (R2914-1)] or the C-terminal [SEPT9 (C) (R2914-2)] region of human SEPT9. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

 SEPT9 Antibody Western (Abiocode)

Fig 1. Western blot of total cell extracts from A) mouse
thymus, B, D) human HeLa, C, E) human Jurkat; using
2 independent Abs against 2 distinct regions of human
SEPT9 at RT for 2 h.