Products

R3142-1 - RHBDF2 (N1) Antibody, Rabbit Polyclonal

Catalog No. R3142-1
Product Name RHBDF2 (N1) Antibody, Rabbit Polyclonal
Price $279
  
 

Quantity: 100 ul                                                                                        Application: WB, IP

Predicted I Observed M.W.: 97 I 90-100 kDa                                          Uniprot ID: Q6PJF5


Background:

RHBDF2 is a multi-pass membrane protein located in endoplasmic reticulum. RHBDF2 is a rhomboid protease-like protein which has no protease activity but regulates the secretion of several ligands of the epidermal growth factor receptor. RHBDF2 indirectly activates the epidermal growth factor receptor signaling pathway and may thereby regulate sleep, cell survival, proliferation and migration.

Other Names:

Inactive rhomboid protein 2, iRhom2, Rhomboid 5 homolog 2, Rhomboid family member 2, Rhomboid veinlet-like protein 5, Rhomboid veinlet-like protein 6, IRHOM2, RHBDL5, RHBDL6

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing N-terminal region of human RHBDF2. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

IP: 1:100-1:200

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data:

RHBDF2 iRhom2 Antibody Western (Abiocode)

Fig 1. A) Western blot of total cell extracts from a. mouse thymus, b. human HeLa, c. human Jurkat; using anti-RHBDF2 (N1) (R3142-1) at RT for 2 h. B) Total extracts from human Jurkat were immunoprecipitated with IgG or anti-RHBDF2 (N1) (R3142-1); followed by WB with the same Ab. C) Western blot of protein extracts from human 293T cells transfected with a vector control (Vec) or Flag-RHBDF2, using indicated Abs at RT for 2 h.

Reference cited for this product:

1. Tang J, Frey JM, Wilson CL, Moncada-Pazos A, Levet C, Freeman M, Rosenfeld ME, Stanley ER, Raines EW, Bornfeldt KE. (2018). Neutrophil and Macrophage Cell Surface Colony-Stimulating Factor 1 Shed by ADAM17 Drives Mouse Macrophage Proliferation in Acute and Chronic Inflammation. Mol. Cell. Biol. 38:1-19.