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R2934-vp - CLTC (vPairTM) Antibodies
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Predicted I Observed M.W.: 192 I 180 kDa Uniprot ID: Q00610 Application: WB Quantity: 50 ul CLTC (N) (R2934-1) Rabbit Polyclonal Antibody & 50 ul CLTC (C) (R2934-2) Rabbit Polyclonal Antibody Product Introduction: vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Background: Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains [provided by RefSeq]. Other Names: Clathrin heavy chain 1, Clathrin heavy chain on chromosome 17, CLH-17, CLH17, CLTCL2, KIAA0034 Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [CLTC (N) (R2934-1)] or the C-terminal [CLTC (C) (R2934-2)] region of human CLTC. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human, Mouse Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. Western blot of total cell extracts from A, C) mousethymus, B, D) human HeLa, E) human Jurkat; using 2 independent Abs against 2 distinct regions of human CLTC at RT for 2 h. |