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R0280-3 - ACTIN (C3) Antibody, Rabbit Polyclonal
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Quantity: 100 ul Application: WB
Predicted I Observed M.W.: 42 kDa Uniprot ID: P60709
Background: Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Beta-Actin (ACTB) encodes one of six different actin proteins, which is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Actins are ubiquitously expressed in all eukaryotic cells.
Other Names:
Actin, cytoplasmic 1, Beta-actin, ACTB, b-Actin, PS1TP5BP1, beta cytoskeletal actin, PS1TP5-binding protein 1
Source and Purity:
Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of human ACTB, which shares approximately 90% homology with Arabidopsis ACTIN2, 3, 4, 7, 8, 11, 12. Antibodies were purified by affinity purification using immunogen.
Storage Buffer and Condition:
Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.
Species Specificity:
Human, Arabidopsis
Tested Applications:
WB: 1:1,000-1:3,000 (detect endogenous protein*)
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.
Product Data:
Fig 1. Western blot of total cell extractsfrom WT arabidopsis, using anti-ACTIN (C3)(R0280-3) at RTfor 2 h. |