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Quantity: 100 ul                                                                                          Application: WB

Predicted I Observed M.W.: 45 I 58 kDa                                                     Uniprot ID: Q9HB65

Background:

RNA polymerase II elongation factor ELL3 is an enhancer-binding elongation factor that specifically binds enhancers in embryonic stem cells (ES cells), marks them, and is required for their future activation during stem cell specification. ELL3 does not only bind to enhancer regions of active genes, but also marks the enhancers that are in a poised or inactive state in ES cells and is required for establishing proper RNA polymerase II occupancy at developmentally regulated genes in a cohesin-dependent manner. ELL3 is probably required for priming developmentally regulated genes for later recruitment of the super elongation complex (SEC), and for transcriptional activation during differentiation.

Other Names:

RNA polymerase II elongation factor ELL3

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing N-terminal region of human ELL3. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: