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Predicted I Observed M.W.: 291 kDa                                                          Uniprot ID: Q9HCK8

Application: WB

Quantity:        50 ul CHD8 (N) (R3237-1) Rabbit Polyclonal Antibody &

                      50 ul CHD8 (C) (R3237-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPair^TM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPair^TM antibodies in the same assay can validate signal specificity since vPair^TM antibodies recognize two independent epitopes of the same protein. Different sets of vPair^TM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Chromodomain-helicase-DNA-binding protein 8 (CHD8) is a DNA helicase that acts as a chromatin remodeling factor and regulates transcription. CHD8 acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. CHD8 suppresses p53-mediated apoptosis by recruiting histone H1 and preventing p53 transactivation activity. CHD8 also acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. In addition, CHD8 acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. CHD8 also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription.

Other Names:

Chromodomain-helicase-DNA-binding protein 8, CHD-8, ATP-dependent helicase CHD8, Helicase with SNF2 domain 1, HELSNF1, KIAA1564

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [CHD8 (N) (R3237-1)] or the C-terminal [CHD8 (C) (R3237-2)] region of human CHD8. Antibodies were purified by affinity purification using immunogen.  

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN₃. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse

Tested Applications:

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: