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R3200-vp - TICAM2 (vPairTM) Antibodies
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Predicted I Observed M.W.: 27, 46 I 27 kDa Uniprot ID: Q86XR7 Application: WB Quantity: 50 ul TICAM2 (M) (R3200-2) Rabbit Polyclonal Antibody & 50 ul TICAM2 (C) (R3200-3) Rabbit Polyclonal Antibody Product Introduction: vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Background: TIR domain-containing adapter molecule 2 (TICAM2) functions as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. TICAM2 physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1; signaling is proposed to occur in early endosomes after endocytosis of TLR4. TICAM2 may also be involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB; however, reports are controversial. Two isoforms of TICAM2 have been identified: isoform 1 (also known as TRAM) and isoform 2 (also known as TAG; TRAM adaptor with GOLD domain), which differ at their N-terminus. Isoform 2 is proposed to inhibit LPS-TLR4 signaling at the late endosome by interaction with isoform 1; thereby disrupting the association of isoform 1 with TICAM1. Other Names: TIR domain-containing adapter molecule 2, TICAM-2, Putative NF-kappa-B-activating protein 502, Source and Purity: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the middle [TICAM2 (M) (R3200-2)] or the C-terminal [TICAM2 (C) (R3200-3)] region of human TICAM2. Antibodies were purified by affinity purification using immunogen. Storage Buffer and Condition: Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt. Species Specificity: Human, Mouse Tested Applications: WB: 1:1,000-1:3,000 (detect endogenous protein*) *: The apparent protein size on WB may be different from the calculated M.W. due to modifications. Product Data: Fig 1. Western blot of total cell extracts fromA) mouse thymus, B, D) human HeLa, C) human Jurkat; using 2 independent Abs against 2 distinct regions of human TICAM2 at RT for 2 h. These 2 Abs predominately recognize TICAM2 isoform 1. |