Abiocode Logo
Products
Contact Us
- Telephone:
1-818-707-0309 - E-Mail:
Abiocode@Abiocode.com
M1084-4a - TET1 (N1) Antibody, Mouse Monoclonal
|
Application: WB, Flow cyt Quantity: 100 ug
Predicted M.W.: 219 kDa Uniprot ID: Q3URK3
Isotype: Mouse IgG1 Clone: 8H10
Background:
TET1 is a methylcytosine dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. TET1 might initiate a process leading to cytosine demethylation through deamination into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. TET1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene silencing induced by cytosine methylation. It has been shown that TET1 plays an important role in embryonic stem cells maintenance and regulating the balance between pluripotency and lineage commitment.
Other Names:
Methylcytosine dioxygenase TET1, CXXC-type zinc finger protein 6, Leukemia-associated protein with a CXXC domain, Ten-eleven translocation 1 gene protein, CXXC6, KIAA1676, LCX
Source and Purity:
Mouse monoclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the N-terminal region of mouse TET1. Antibodies were purified by Caprylic Acid- Ammonium Sulfate precipitation.
Storage Buffer and Condition:
Supplied in 1 x PBS (pH 7.4), 40% Glycerol, 0.02% Thimerosal. Store at -20 °C. Stable for 6 months from date of receipt.
Tested Applications:
WB: 1:1,000-1:3,000 (detect endogenous protein*)
Flow cytometry: 1:100-1:400
*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.
Species Specificity:
Mouse
Product Data:
Fig 1. (A) Western blot of total cell extracts from human 293 T cells transfected with the empty vector, or Flag-tagged murine TET1, TET2 or TET3; using indicated Abs at RT for 2h. (B) Western blot of total cell extracts from mouse brain; using anti-TET1 (N1) (M1084-4a). (C) Flow cytometry was performed with mouse thymocytes, using PE-labeled 2’ anti-mouse IgG only (black line), or anti-TET1 (N1) (M1084-4a) followed by 2’ anti-mouse IgG-PE (red line).
|
