Abiocode Logo

Predicted I Observed M.W.: 70 kDa                                                      Uniprot ID: Q96LB3

Application: WB

Quantity:        50 ul IFT74 (N) (R3219-1) Rabbit Polyclonal Antibody &

                        50 ul IFT74 (C) (R3219-2) Rabbit Polyclonal Antibody 

Product Introduction:

vPairTM antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Abiocode to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody-based assays. The use of a pair of fully characterized vPairTM antibodies in the same assay can validate signal specificity since vPairTM antibodies recognize two independent epitopes of the same protein. Different sets of vPairTM antibodies are developed at Abiocode to work with specific applications, including antibody arrays, Western blot, IP-Western, ChIP, IHC, and FACS.

Background:

Intraflagellar transport protein 74 homolog (IFT74) is a component of the intraflagellar transport (IFT) complex B: together with IFT81, IFT74 forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. IFT74 binds to beta-tubulin via its basic region, and is required for ciliogenesis. 

Other Names:

Intraflagellar transport protein 74 homolog, Capillary morphogenesis gene 1 protein, CMG-1, Coiled-coil domain-containing protein 2, CCDC2, CMG1                                                                  

Source and Purity:

Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal [IFT74 (N) (R3219-1)] or the C-terminal [IFT74 (C) (R3219-2)] region of human IFT74. Antibodies were purified by affinity purification using immunogen. 

Storage Buffer and Condition:

Supplied in 1 x PBS (pH 7.4), 100 ug/ml BSA, 40% Glycerol, 0.01% NaN3. Store at -20 °C. Stable for 6 months from date of receipt.

Species Specificity:

Human, Mouse 

Tested Applications: 

WB: 1:1,000-1:3,000 (detect endogenous protein*)

*: The apparent protein size on WB may be different from the calculated M.W. due to modifications.

Product Data: